{"in": ["medium"], "out": "the cells"}
{"in": ["PBS/BSA"], "out": "FITC-conjugated anti-CD4 CD25"}
{"in": ["FITC-conjugated anti-CD4 CD25"], "out": "anti-FITC microbeads"}
{"in": ["cells"], "out": ""}
{"in": ["anti-FITC microbeads"], "out": "release reagent"}
{"in": [], "out": ""}
{"in": ["release reagent (available"], "out": "CD4+CD25+ Tregs"}
{"in": ["\u03b1-biotin microbeads"], "out": "the purified CD4T cell suspension"}
{"in": ["CD25cells"], "out": "CD4+CD25&#x2013;"}
{"in": ["3\u00d710^4 irradiated (3000 Rad) T cell-depleted splenocytes"], "out": ""}
{"in": ["Treg Tcon cells", "responders"], "out": "CFSE+ responders"}
{"in": ["CFSE+ responders"], "out": "forward scatter"}
{"in": ["CD4+CD25&#x2013;"], "out": "cell death"}