{"action": "make", "output": "", "container": ["the abdomen"]}
{"action": "handle", "output": "", "container": ["<<<the mesentery>>>"], "reagent": [""]}
{"action": "maintain", "output": "continuous saline superfusion", "temperature": ["<<<37 \u00baC>>>"]}
{"action": "repeat", "output": "leukocyte adhesion", "reagent": [""]}
{"action": "ensure", "output": "", "reagent": ["no leukocyte adhesion"], "time": [""]}
{"action": "induce", "output": "", "time": ["<<<10 min>>>"], "reagent": ["ischemia"], "device": ["ligating the anterior mesenteric artery"]}
{"action": "cut", "output": "blood flow", "device": ["the snare"]}
{"action": "repeat", "output": "the ligation steps", "reagent": ["blood flow"]}
{"action": "observe", "output": "", "device": ["LOSOM"], "reagent": ["<<<microcirculation>>>"]}