{"action": "dissect", "output": "", "reagent": ["tissues"]}
{"action": "patch", "output": "", "reagent": ["<<<4% PFA>>>"], "time": ["4 h"], "temperature": ["<<<room temperature>>>"]}
{"action": "dehydrate", "output": "", "reagent": ["30% sucrose solution"]}
{"action": "prepare", "output": "", "device": ["Cryostat microtome"], "container": ["thin tissue sections"]}
{"action": "wash", "output": "samples", "reagent": ["0.3% Triton X-100 in PBS (PBST)"]}
{"action": "wash", "output": "HCR amplification buffer", "reagent": ["samples"]}
{"action": "incubate", "output": "secondary antibodies", "reagent": ["HCR amplification buffer"], "temperature": [""], "time": [""]}
{"action": "detect", "output": "samples", "reagent": ["secondary antibodies"]}
{"action": "wash", "output": "", "reagent": ["samples"]}
{"action": "add", "output": "GO", "container": ["amplification buffer"], "concentration": ["12.5 nM"], "reagent": [""]}
{"action": "add", "output": "samples", "reagent": ["GO"], "concentration": ["<<<20 \u00b5g/mL>>>"]}
{"action": "wash", "output": "", "reagent": ["samples"]}