{"action": "add", "output": "", "reagent": ["Chloroform:Methanol:Isopropanol (2:1:1)"], "container": ["plasma"]}
{"action": "discard", "output": "the supernatant", "container": [""]}
{"action": "add", "output": "", "volume": ["<<<800 \u00b5l>>>"], "reagent": ["TCA/Acetone (50%)"], "device": [""]}
{"action": "incubate", "output": "", "temperature": ["-20\u00b0"]}
{"action": "discard", "output": "the supernatant", "container": [""]}
{"action": "add", "output": "", "reagent": ["<<<40% Ammonium sulfate>>>"]}
{"action": "incubate", "output": "", "temperature": ["ice"], "time": ["<<<30 minutes>>>"]}
{"action": "remove", "output": "the supernatant", "container": [""]}
{"action": "clean", "output": "the pellet", "device": ["Bio-Rad clean-up kit"]}
{"action": "dissolve", "output": "", "volume": ["150-250 \u00b5l"], "reagent": ["<<<Rehydration buffer>>>"]}
{"action": "resolve", "output": "", "concentration": ["10%"], "container": ["SDS-PAGE"], "length": [""], "reagent": ["<<<the pellet>>>"]}