{"action": "mix", "output": "Ficoll-Hypaque", "reagent": ["fresh EDTA blood", "room-temperature PBS"], "container": ["conical centrifuge tube"], "temperature": ["room temperature"]}
{"action": "layer", "output": "", "reagent": ["<<<Ficoll-Hypaque>>>"], "volume": ["3 ml"], "container": ["the blood/PBS mixture"]}
{"action": "centrifuge", "output": "", "force": ["<<<900 x g>>>"], "time": ["<<<30 minutes>>>"], "temperature": ["<<<room temperature>>>"]}
{"action": "remove", "output": "the upper plasma layer", "device": [""]}
{"action": "transfer", "output": "", "container": ["the mononuclear cell layer"]}
{"action": "rinse", "output": "", "reagent": ["<<<PBS>>>"], "times": ["three times"], "volume": ["3x volume of the cell layer"], "force": ["<<<400 x g>>>"], "time": ["<<<10 minutes>>>"]}
{"action": "resuspend", "output": "", "container": ["the cells"], "reagent": [""], "volume": [""]}