{"action": "clarify", "output": "", "device": [""]}
{"action": "incubate", "output": "", "temperature": ["37 \u00b0C"], "concentration": ["5% CO2"], "time": ["24 h"]}
{"action": "maintain", "output": "no HSV1-TK", "temperature": ["37 \u00b0C"], "time": ["<<<3 h>>>"]}
{"action": "keep", "output": "control", "reagent": ["no HSV1-TK"], "temperature": [""]}
{"action": "remove", "output": "", "container": ["the medium"]}
{"action": "rinse", "output": "", "temperature": ["ice-cold"], "time": [""], "reagent": ["<<<PBS>>>"]}
{"action": "drain", "output": "the remaining total activities", "time": [""]}
{"action": "add", "output": "", "reagent": ["0.1 N NaOH"], "container": ["each well"]}
{"action": "leave", "output": "cell lysate", "temperature": ["<<<room temperature>>>"]}
{"action": "add", "output": "", "reagent": ["cell lysate", "1x Bradford protein assay reagent"], "volume": ["20 \u03bcL", "1 mL"]}
{"action": "read", "output": "the absorbance", "device": ["<<<a spectrophotometer>>>"], "length": [""]}
{"action": "use", "output": "", "reagent": ["cell lysate"]}
{"action": "transfer", "output": "3H-Penciclovir", "volume": ["the remaining cell lysate (480 \u03bcL)"], "container": ["a scintillation vial"]}
{"action": "provide", "output": "a negative control", "reagent": ["3H-Penciclovir"]}
{"action": "passage", "output": "3H-Penciclovir", "reagent": ["each sample"], "container": ["the pooled medium"], "length": [""]}
{"action": "evaluate", "output": "different samples", "reagent": ["3H-Penciclovir"]}
{"action": "passage", "output": "an average reading/min", "reagent": ["different samples"]}
{"action": "normalize", "output": "3H-PCV", "concentration": ["<<<protein concentration>>>"]}
{"action": "confirm", "output": "3H-PCV", "reagent": ["3H-PCV"]}
{"action": "keep", "output": "protein/total", "reagent": ["3H-PCV"], "temperature": [""]}