{"action": "grow", "output": "one tumor", "reagent": ["Lewis Lung Cancer (LLC)"]}
{"action": "place", "output": "", "reagent": ["one tumor"], "container": ["a 100 mm Petri dish"]}
{"action": "add", "output": "tumors", "volume": ["5-10 ml HBSS"], "temperature": ["<<<RT>>>"]}
{"action": "mince", "output": "tumor tissue suspension", "reagent": ["tumors"], "container": ["<<<a 5 ml pipette>>>"]}
{"action": "transfer", "output": "", "reagent": ["<<<tumor tissue suspension>>>"], "container": ["50 ml non-vented tissue culture flasks"]}
{"action": "rinse", "output": "", "volume": ["up to 40 ml"]}
{"action": "transfer", "output": "", "container": ["the suspension"]}
{"action": "add", "output": "", "volume": ["<<<5 ml>>>"], "container": ["<<<the flask>>>"]}
{"action": "shake", "output": "", "temperature": ["RT"], "device": ["<<<shaker>>>"], "speed": ["<<<80 rpm>>>"], "time": ["1-3 h"]}
{"action": "centrifuge", "output": "cells", "temperature": ["RT"], "time": ["<<<10 min>>>"], "force": ["<<<50 x g>>>"]}
{"action": "collect", "output": "the supernatant", "container": [""]}
{"action": "discard", "output": "", "container": ["the tube"]}
{"action": "centrifuge", "output": "the supernatant", "force": ["200 x g"], "time": ["5 min"]}
{"action": "suspend", "output": "", "reagent": ["cell pellet"], "volume": ["2 ml"], "time": ["<<<1 min>>>"]}
{"action": "add", "output": "cells", "reagent": ["more ACK lysing buffer"]}
{"action": "re-suspend", "output": "", "reagent": ["<<<cells>>>"], "volume": ["15 ml"]}
{"action": "add", "output": "wash buffer", "container": ["<<<a 50 ml tube>>>"], "reagent": ["cells"]}
{"action": "collect", "output": ""}
{"action": "wash", "output": "", "reagent": ["wash buffer"]}
{"action": "centrifuge", "output": "tumor", "temperature": ["RT"], "time": ["10 min"], "force": ["<<<425 x g>>>"]}
{"action": "note", "output": "", "device": ["centrifugation"], "reagent": ["tumor"]}
{"action": "re-suspend", "output": "", "reagent": ["cells"], "device": ["<<<flow cytometry>>>"]}
{"action": "see", "output": ""}
{"action": "see", "output": ""}