{"action": "dilute", "output": "", "reagent": ["blood", "PBS"]}
{"action": "collect", "output": "interphase", "reagent": ["WBC"], "device": ["Pasteur pipette"]}
{"action": "pellet", "output": "", "reagent": ["<<<cells>>>"], "speed": ["<<<300 x g>>>"], "time": ["<<<10 min>>>"], "temperature": ["RT"]}
{"action": "add", "output": "", "volume": ["50 ml"], "reagent": ["<<<PBS>>>"]}
{"action": "take", "output": "FACs analysis", "volume": [""]}
{"action": "centrifuge", "output": "", "speed": ["300 x g"], "time": ["10 min"], "temperature": ["RT"]}
{"action": "suspend", "output": "", "reagent": ["<<<pellet>>>"], "volume": ["300 \u00b5l"]}
{"action": "incubate", "output": "", "temperature": ["<<<4 \u00b0C>>>"], "time": ["10 min (RT)"]}
{"action": "charge", "output": "", "device": ["<<<LS column>>>"], "reagent": ["<<<MACS buffer>>>"], "volume": ["3 ml"]}
{"action": "re-suspend", "output": "", "reagent": ["cells"], "volume": ["500 \u00b5l"]}
{"action": "count", "output": "", "reagent": ["cells"]}
{"action": "pellet", "output": "", "reagent": ["cells"], "speed": ["<<<400 x g>>>"], "time": ["<<<5 min>>>"]}
{"action": "incubate", "output": "directly conjugated", "time": ["10 min"], "temperature": ["<<<RT>>>"]}
{"action": "add", "output": "", "reagent": ["directly conjugated antibody"]}
{"action": "suspend", "output": "", "volume": ["500 \u00b5l"]}
{"action": "proceed", "output": "", "device": ["FACS analysis"]}