{"action": "determine", "output": "cell count", "device": ["Casy cell counter"]}
{"action": "mix", "output": "Fugene\u00ae HD solution", "volume": ["<<<25 \u00b5l>>>"], "mass": ["0.5 \u00b5g HyPerER"]}
{"action": "incubate", "output": "", "reagent": ["Fugene\u00ae HD solution"], "temperature": ["<<<room temperature>>>"], "time": ["15 min"]}
{"action": "add", "output": "cells", "container": ["<<<cells>>>"], "reagent": ["cell count"]}
{"action": "add", "output": "", "volume": ["2.6 ml"], "reagent": ["<<<growth medium>>>"]}
{"action": "incubate", "output": "", "reagent": ["cells"], "temperature": ["<<<37\u00b0C>>>"], "time": ["<<<24-48 h>>>"]}
{"action": "perform", "output": "culture medium", "device": ["Olympus Fluoview 3000 microscope"]}
{"action": "remove", "output": "pre-warmed HEPES Imaging Buffer", "reagent": ["<<<culture medium>>>"]}
{"action": "rinse", "output": "", "volume": ["1 ml"], "reagent": ["pre-warmed HEPES Imaging Buffer"]}
{"action": "add", "output": "stimulating substances (e.g., thapsigargin)", "volume": ["1 ml HEPES Imaging Buffer"], "device": ["microscope stage"], "reagent": [""]}
{"action": "add", "output": "baseline oxidative status", "reagent": ["stimulating substances (e.g., thapsigargin)"]}
{"action": "proceed", "output": "measurement", "reagent": ["<<<100 \u00b5M H2O2>>>"]}