{"action": "transfect", "output": "stable clones", "reagent": ["ISX fusion GFP expression"]}
{"action": "generate", "output": "", "reagent": ["<<<clones>>>"]}
{"action": "prepare", "output": "", "concentration": ["0.6% agar"]}
{"action": "keep", "output": "", "temperature": ["<<<65\u00b0C>>>"]}
{"action": "repeat", "output": "the preparation steps", "time": [""]}
{"action": "dilute", "output": "", "concentration": ["1 x 10^4 cells/ml", "20% FBS"]}
{"action": "mix", "output": "", "concentration": ["0.6% agar"], "reagent": ["stable clones"]}
{"action": "add", "output": "the medium addition", "volume": ["2 ml"], "concentration": ["<<<10% FBS MEM culture medium>>>"]}
{"action": "repeat", "output": "", "reagent": ["the medium addition"]}
{"action": "repeat", "output": "the staining process", "time": [""]}