{"action": "spin", "output": "", "speed": ["<<<500-1,000 x g>>>"], "time": ["<<<10 min>>>"]}
{"action": "add", "output": "", "volume": ["4 ml XBP buffer"], "reagent": ["supernatant"]}
{"action": "add", "output": "flow-through", "reagent": ["sample/XBP mix"], "container": ["exoEasy maxi spin column"]}
{"action": "centrifuge", "output": "Qiazol", "volume": ["10 ml XWP"], "time": ["5 min"], "speed": ["<<<5,000 x g>>>"], "device": ["<<<spin column>>>"]}
{"action": "add", "output": "", "reagent": ["<<<Qiazol>>>"]}
{"action": "add", "output": "", "reagent": ["flow-through"], "container": ["PLG tube"]}
{"action": "add", "output": "", "volume": ["90 \u00b5L chloroform"]}
{"action": "shake", "output": "", "time": ["<<<15 s>>>"], "temperature": ["<<<RT>>>"]}
{"action": "transfer", "output": "upper aqueous phase", "container": ["new tube"]}
{"action": "add", "output": "", "volume": ["2 volumes"], "reagent": ["upper aqueous phase"]}
{"action": "centrifuge", "output": "", "container": ["MinElute spin column"], "time": ["<<<15 s>>>"], "speed": ["<<<1,000>>>"]}
{"action": "centrifuge", "output": "", "volume": ["700 \u00b5L"], "time": ["15 s"], "speed": [">=8,000"]}
{"action": "centrifuge", "output": "RNase-free", "volume": ["500 \u00b5L Buffer RPE"], "time": ["15 s"], "speed": [">=8,000"]}
{"action": "add", "output": "RNA", "reagent": ["<<<RNase-free>>>"]}
{"action": "centrifuge", "output": "", "reagent": ["RNA"], "speed": [""], "time": [""]}