{"action": "make", "container": ["the abdomen"], "output": ""}
{"action": "handle", "container": ["the mesentery"], "output": "", "reagent": [""]}
{"action": "maintain", "output": "continuous saline superfusion", "temperature": ["37 \u00baC"]}
{"action": "repeat", "output": "leukocyte adhesion", "reagent": [""]}
{"action": "ensure", "output": "", "reagent": ["no leukocyte adhesion"], "time": [""]}
{"action": "induce", "device": ["ligating the anterior mesenteric artery"], "output": "", "reagent": ["ischemia"], "time": ["10 min"]}
{"action": "cut", "device": ["the snare"], "output": "blood flow"}
{"action": "repeat", "output": "the ligation steps", "reagent": ["blood flow"]}
{"action": "observe", "device": ["LOSOM"], "output": "", "reagent": ["microcirculation"]}