{"action": "transfer", "container": ["a glass centrifuge vial"], "output": "", "reagent": ["the sample (plasma, , cell suspension)"]}
{"action": "modify", "device": [""], "length": [""], "output": "heparinized blood.1 ml medium", "volume": ["1 ml"]}
{"action": "take", "output": "", "reagent": ["heparinized blood.1 ml medium"]}
{"action": "take", "output": "a plasma sample", "reagent": [""]}
{"action": "add", "output": "", "reagent": ["18.5% HCl"], "volume": ["300 \u00b5l"]}
{"action": "show", "output": "step A7", "reagent": ["a plasma sample"]}
{"action": "extract", "container": ["the upper aqueous phase"], "output": "the CHCl3", "reagent": ["step A7"]}
{"action": "add", "output": "", "reagent": ["this CHCl3-phase"]}
{"action": "rinse", "output": "", "reagent": ["the CHCl3"], "temperature": ["60 \u00b0C"], "time": ["45 min"]}
{"action": "dry", "output": "", "reagent": ["samples"], "time": ["1-20 min"]}
{"action": "equilibrate", "concentration": ["90% solution"], "output": "S1P", "volume": [""]}
{"action": "examine", "output": "quantitative analysis", "reagent": ["S1P"]}