{"action": "collect", "container": ["RPMI 1640 media (no FBS)"], "output": ""}
{"action": "treat", "concentration": ["100 ng/ml PTX"], "output": ""}
{"action": "rotate", "output": "", "temperature": ["37\u00b0C"], "time": ["3-4 h"]}
{"action": "protect", "container": ["tubes"], "output": ""}
{"action": "rotate", "output": "", "temperature": ["37\u00b0C"], "time": ["30 min"]}
{"action": "repeat", "output": "the staining steps", "reagent": [""]}
{"action": "induce", "concentration": ["50 \u00b5l 10"], "output": "", "reagent": ["ionomycin"], "time": ["3 min"]}
{"action": "repeat", "output": "the stimulation", "reagent": ["the staining steps"]}
{"action": "note", "bool": [""], "output": "", "reagent": ["SDF-1", "other chemokines"]}