Introduce a novel C57BL/6 mouse (8–16 weeks old) into the home cage of single-housed CD1 mouse (4–6 months old).

Repeat the procedure for three consecutive days, using different C57BL/6 mouse for each CD1 mouse each day.
{"action": "repeat", "output": "", "time": ["three consecutive days"]}
Resect the gonads of selected CD1 aggressors (refer to Sophocleous ) using surgical scissors , to avoid harmful behaviors against experimental mice in subsequent experiments. #####%%%%% output "harmful behaviors" are used in line10
{"action": "resect", "output": "harmful behaviors", "device": ["surgical scissors"]}
Reuse CD1 aggressors for different pup litters for no longer than three months.

Randomly assign mouse pup litters to control at postnatal day 15 (PD 15).
{"action": "assign", "output": "", "reagent": ["harmful behaviors"]}
Introduce each pup of the experimental group into the home cage of an unfamiliar CD1 aggressor for a session of 30 min.
{"action": "introduce", "output": "", "time": ["30 min"]}
Place the pup litters back in their home cages with dams.

Repeat the aggression with different resident home cages every day from PD 15 to PD 24.
{"action": "repeat", "output": "", "time": ["PD 15"]}
Keep pups in the control group undisturbed from PD 15 to PD 24.
{"action": "keep", "output": "", "time": ["PD 15"]}
Raise all experimental mice individually in their cages after the 10-day social defeat for a month.
{"action": "raise", "output": "", "time": ["10-day"], "reagent": [""]}
Conduct the SIT on PD 55 between 10:00 .

Analyze the time that experimental mice spend in the social interaction with EthoVision XT.
{"action": "evaluate", "output": "", "device": ["EthoVision XT"], "reagent": [""]}
Remove the experimental mice from the arena .
{"action": "remove", "output": "", "container": ["the experimental mice"], "volume": [""]}
place them into their home cage for 1 min #####%%%%% output "experimental mice" are used in line30
{"action": "place", "output": "experimental mice", "container": ["them", "their home cage"]}
Introduce experimental mice into the arena for another 300 s. 7.
{"action": "introduce", "output": "", "reagent": ["experimental mice", ""], "container": ["the arena"], "device": [""]}
Analyze the time that experimental mice spend in the social interaction zone with EthoVision XT.
{"action": "evaluate", "output": "", "device": ["EthoVision XT"], "reagent": [""]}
Clean the arena with odorless cleaning solution .
{"action": "clean", "output": "", "container": ["the arena"]}
Conduct the OFT on PD 56 between 10:00 .

Introduce each experimental mouse into the arena for 10 min.
{"action": "introduce", "output": "", "time": ["10 min"]}
Analyze the time that experimental mice spend in the central zones of the open field arena with EthoVision XT.
{"action": "evaluate", "output": "", "device": ["EthoVision XT"], "reagent": [""]}
Analyze the total distance traveled by experimental mice with EthoVision XT.
{"action": "evaluate", "output": "", "container": ["experimental mice"], "reagent": [""]}
Clean the arena with odorless cleaning solution .
{"action": "clean", "output": "", "container": ["the arena"]}