Place wheat seeds in a 94 mm diameter Petri dish plate containing 2 filter papers .
{"action": "place", "output": "", "container": ["2 filter papers"]}
Different leaf sections are placed in a square Petri dish with their cut ends held between an upper section.
{"action": "put", "output": "", "container": ["square Petri dish"]}
Put the cut ends between a sandwich of 1 % plant agar pH 5.7 containing 0.5 mM benzimidazole (Figure 1B, Video 1).
{"action": "put", "output": "", "device": [""]}
Note: Removing the agar from the center of the plate prevent excessive fungal growth at the point of leaf inoculation, additionally removed agar section that could be used to form the sandwich. #####%%%%% output "excessive fungal growth" are not used
{"action": "note", "output": "excessive fungal growth", "volume": [""]}
Benzimidazole is used to delay the leaf senescence.<p>imgsrc:https://bio-protocol.org/javascript:;</p> Video 1. #####%%%%% output "the leaf" are not used
{"action": "use", "output": "the leaf", "reagent": ["benzimidazole"]}
Placement of the leaf cut ends between agar sections . #####%%%%% output "the leaf cut" are not used
{"action": "start", "output": "the leaf cut", "volume": [""]}
Puncture the center of each leaf section with the tip of a glass Pasteur pipette #####%%%%% output "0" are used in line16
{"action": "puncture", "output": "0", "container": ["each leaf section"], "volume": [""]}
treat with a 4 μl droplet of 0
{"action": "treat", "output": "", "reagent": ["0"]}
The level of condensation inside your plates may differ .

affect reproducible disease development

Analyse the leaf sections at 4 days post-inoculation (Figure 2).Note:
{"action": "analyse", "output": ""}
In that case photograph your leaf section every day.
{"action": "photograph", "output": ""}
Repeat the experiment at least three times, each time including 6 plates per treatment, .
{"action": "repeat", "output": ""}